Editing the immune system in vivo in mice using CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing of transplanted hematopoietic stem cells

•Efficient method to deliver CRISPR/Cas9 system in mouse LSK cells.•Cost-effective to produce mice bearing genetic edits in hematopoietic cells including immune cells.•Not restricted to Cas9 knock-in strains of mice, so potentially compatible with more mouse models.