Manual and automated tissue segmentation confirm the impact of thalamus atrophy on cognition in multiple sclerosis: A multicenter study

•Thalamus atrophy is associated with cognitive impairment in multiple sclerosis.•This was confirmed by automated and manual segmentations, but effect sizes varied.•The algorithms work in a multi-center setting.•Automated techniques exhibit proportional bias with respect to thalamus size.•Differences between vendors can affect the robustness of these associations.