Investigation of fennel protein extracts by shot-gun Fourier transform ion cyclotron resonance mass spectrometry

•Rapid shot-gun method by FT-ICR-MS for characterization of fennel proteins.•Custom-made non-redundant fennel-specific proteome database.•In silico generation of peptide m/z ratios from digested fennel proteins and MATLAB search.•Peptide Mass Fingerprint database searching for fennel protein characterization.•Starting point for the assessment of the pattern of allergenic components in fennel.