Cucumopine type Agrobacterium rhizogenes K599 (NCPPB2659) T-DNA mediated plant transformation and its application.

Agrobacterium rhizogenes infects plants to induce hairy roots. It is classified into four different types such as agropine, mannopine, cucumopine and mikimopine. A. rhizogenes K599, also known as NCPPB2659, is a representative strain of the cucumopine type. In this review, we discussed the functions of 11 genes on the T-DNA of Ri plasmid pRi2659 in K599. We summarized the infectivity of K599 on a variety of plants and reviewed the applications of K599-induced hairy roots. These applications include analyses of gene and recombinant plasmid functions, direct employment as root parasite host and model system for the interaction between root and its parasite, production of herbal active ingredients as bioreactor, and the regeneration of transgenic plant. We also prospected the application potentials of A. rhizogenes K599 in transgenic plant and the mechanism of K599-mediated transgenic plant.