Targeted quantification of B vitamins using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry in faba bean seeds

•The first report of a comprehensive quantification of 16 B vitamers in faba bean.•UPLC-SRM MS was used for accurate quantification with a short analysis time.•Significant genotype × environment interaction was observed for most B vitamins.•Low and high tannin faba beans showed different B vitamin profiles.•Faba bean is proved to be a good source of water soluble B vitamins.