Comparative study of oxidative stress between ovsynch and doublesynch estrus synchronization protocols on the day of AI in cyclic murrah buffaloes during winter

The aim of present study was to compare the effect on oxidative stress parameters on the day of AI after synchronizing estrous of cyclic Murrah Buffalo with Ovsynch and Doublesynch protocols during winter. Forty three cyclic Murrah buffaloes were synchronized for estrus with standard Ovsynch (n=18) and Doublesynch protocol (n=25) and inseminated fixed time at 8 and 24hr of last GnRH injection. Blood samples were analyzed for the level of oxidative stress parameters. Overall, there was no significant (P\u003c0.05) difference found in blood plasma concentrations of MDA, GSH-Px and SOD day of AI. In conclusion, the synchronization protocols do not impact on oxidative stress parameters in cyclic Murrah buffaloes.