Effect of glutathione on viability and progressive motility of Hariana bull spermatozoa during cryopreservation in semi-arid region

The present study was conducted to evaluate the effect of Glutathione, an anti-oxidant, on the sperm viability and progressive moltility of bovine bull spermatozoa at different stages of semen preservation (After dilution, pre-freeze) and subsequently at post thaw. Following evaluation of fresh semen and those which confirmed the standard as stated vide supra, the semen sample of each bull were split into three equal parts and extended with three different combination of dilutors i.e. Glycerolated Egg Yolk Tris (GEYT) (control), GEYT with 0.5mM Glutathione (T1) and GEYT with 1.0mM Glutathione (T2). These samples were processed for cryopreservation and thawed thereafter. The mean percentage of sperm viability differes significantly (P\u003c0.05) between treatment and control group at post-thaw and it was 56.60±0.69, 69.71±0.33 and 64.24±0.52 percent in control, T1 and T2 respectively. Mean percentage of post thaw progressive motility also differed significantly (P\u003c0.05) between treatment and control group and it was 47.79±0.65, 60.08±0.64 and 55.21±0.65 percent in control, T1 and T2 respectively. Comparison (Unpaired‘t’ test) between the bulls did not revealed any significant difference.