Mutation in the BRACA2 gene and change of tissue architecture in canine mammary tumour

Blood samples were collected with anticoagulant from 20 female dogs brought for treatment of mammary tumour in College of Veterinary Science, AAU, Khanapara. DNA was isolated from blood and amplified BRCA2 (Exon 27) showing the mutation of adenine to cytosine was confirmed as cancer positive samples. Histopathological sections prepared from tumour positive mammary tissues revealed the cartilaginous structures around mammary ducts, ductules filled with fat globules, marked hypertrophy and presence of inflammatory cells in the endothelial lining of blood vessels, hypertrophy and hyperplasia of epithelial lining of mammary alveoli. From the present study it can be concluded that a massive changes of tissue architecture is occur in canine mammary tumour.