Changes Of Gut Microbiota In Fap And Uc That Might Harbor Colorectal Cancer Carcinogenesis

Inflammatory bowel diseases, Familial adenomatous polyposis(FAP) and colorectal cancer(CRC) are associated with alterations of the intestinal microbiota. However, few data are available on the perpetuation of FAP and Ulcerative Colitis(UC) in relation to microbial dysbiosis. This study evaluated the UC and genetically confirmed FAP patients9 gut microbial balance in concordance to clinical outcome. Fecal materials (average mass of 0.54 g) were collected from three FAP and five UC patients to compare with healthy individuals as control group. Genomic materials of microbiota were isolated via QIAamp PowerFecal DNA kit according to manufacturer instructions. Next generation sequencing of 16S rRNA was performed by using QIAseq 16S/ITS panel in Illumina Miseq Platform. Data processing and bioinformatics analysis were performed via CLC Genomic Workbench bioinformatics tool. The comparison between FAP subjects, UC patients and control group revealed an alteration in the intestinal microbial composition. More in details, relative abundance of class levels showing statistical significance differences among FAP, UC and control group was given in Table 1, in which the sample groups are reported in column while class levels are in row. Our preliminary data focused on the explanation of how dysbiosis can lead to inflammation and drive processes together with host genetic profile that leads to colorectal carcinogenesis. Citation Format: Atil Bisgin, Abdullah Hanta, Ahmet Rencuzogullari, Ibrahim Boga. Changes of gut microbiota in FAP and UC that might harbor colorectal cancer carcinogenesis [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 3346.