Transcriptome-wide effect of Salix SmSPR1 in etiolated seedling of Arabidopsis
Journal of Forestry Research(2020)
摘要
Microtubules and their regulatory proteins are involved in the regulation of plant cell morphology. SPIRAL1 (SPR1), a plant-specific microtubule-binding protein, is critical in regulating the anisotropic growth of plant cells. Our previous study showed that overexpressed Salix SmSPR1 genes in Arabidopsis thaliana caused right-handed spiral elongation in etiolated seedlings, but there were no morphological differences between wild-type and transgenic seedlings under varied light conditions. We then studied the transcriptional regulation patterns in transgenic plants engineered with the SmSPR1 gene. Transcriptomic results showed that a large number of differentially expressed genes were involved in plant light signal reception, chlorophyll synthesis and photosystem structure. Eleven gene families with 42 photosynthesis-related genes and 6 light-responsive genes were involved in regulation of cell morphology. Our results showed that these genes in the SmSPR1-ox line were particularly down-regulated under dark conditions. In addition, 33 TFs showed differences between SmSPR1-ox and wild-type lines. Taken together, the transcriptome analysis provides new insight into investigating the molecular mechanisms of light-induced cell morphological changes mediated by the microtubule binding protein SPR1.
更多查看译文
关键词
Arabidopsis thaliana
, Cell anisotropism,
SmSPR1
, Transcription factor, Transcriptome analysis
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要