Silver Decorated Green Nanocolloids As Potent Antibacterial And Antibiofilm Agent Against Antibiotic Resistant Organisms Isolated From Tannery Effluent

Pollution is caused because of poor disposal of solid and liquid waste from the leather industry and release 20% of treated effluent and while the rest of 80% is untreated. The tannery effluent is a house for many antibiotic resistant bacteria and multi-drug resistant bacteria and these bacteria cause various bacterial infections which cannot be treated by antibiotics. This current work is focused on isolation and identification of antibiotic resistant strains from tannery effluent. To eliminate the antibiotic resistant strains from tannery effluent and make it beneficial to the society, green nanocolloid was synthesized from traditional medicinal plantAndographis paniculata. Various physio-chemical characterizations such as UV-Vis spectrum, FT-IR spectrum, Particle size analysis, Zeta potential analysis, FE-SEM and elemental analysis using EDAX were employed to identify the characteristic nature of green silver nanocolloids. Antibacterial and antibiofilm assays were performed to validate the efficacy of green nanocolloids. The results showed that ApNc had potent Antibacterial and antibio-film activity against antibiotic resistant strains isolated from tannery effluent. These green nanocolloids could be the alternate source to eradicate antibiotic resistant strains thriving in the tannery effluent. In future this formulation can be employed as a nanospary to avoid the spread of antibiotic resistant strains entering into human and animal system. This research work has been undertaken for the benefit of the society to provide people with better resources and to establish an environment that is disease free and eco-friendly.