The composition analysis of free and bonded fatty acids in Polysorbate 80 by on-line two-step pyrolytic methylation-gas chromatography

Zhongping Huang,Chaochao Tu,Huijun Liu,Lili Wang,Zuoyi Zhu,Ichi Watanabe

Microchemical Journal（2020）

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摘要

•On-line two-step pyrolytic methylation technique combined with gas chromatography.•Composition analysis of free and bonded fatty acids in Polysorbate 80 respectively.•Free fatty acids were converted to methyl esters by tetramethylammonium acetate.•Bonded fatty acids were converted to methyl esters by tetramethylammonium hydroxide.

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关键词

On-line two-step pyrolytic methylation technique,Polysorbate 80,Free fatty acids,Bonded fatty acids

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