Polo-Like Kinase 1 Independently Controls Microtubule-Nucleating Capacity And Size Of The Centrosome
JOURNAL OF CELL BIOLOGY(2021)
摘要
Centrosomes are composed of a centriolar core surrounded by a pericentriolar material (PCM) matrix that docks microtubulenucleating gamma-tubulin complexes. During mitotic entry, the PCM matrix increases in size and nucleating capacity in a process called centrosome maturation. Polo-like kinase 1 (PLK1) is recruited to centrosomes and phosphorylates PCM matrix proteins to drive their self-assembly, which leads to PCM expansion. Here, we show that in addition to controlling PCM expansion, PLK1 independently controls the generation of binding sites for.-tubulin complexes on the PCM matrix. Selectively preventing the generation of PLK1-dependent gamma-tubulin docking sites led to spindle defects and impaired chromosome segregation without affecting PCM expansion, highlighting the importance of phospho-regulated centrosomal.-tubulin docking sites in spindle assembly. Inhibiting both.-tubulin docking and PCM expansion by mutating substrate target sites recapitulated the effects of loss of centrosomal PLK1 on the ability of centrosomes to catalyze spindle assembly.
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关键词
centrosome,polo-like,microtubule-nucleating
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