Determination of a Novel Antimycobacterial Agent in a Single Drop of a Solution by Voltammetry at a Glassy Carbon Electrode

One of the novel antimycobacterial agents from the group of hydroxynaphthalene carboxamides (namely I-hydroxy-X-(4-nitrophenyl)naphthalene-2-carboxamide) was investigated using voltammetric methods for the first time. The study was carried out at a glassy carbon electrode by methods of cathodic and anodic differential pulse voltammetry in micro volumns (20 mu L) of an aqueous solution at pH 7 containing 10% dimethyl sulfoxide and in a real matrix of a bacterial growth medium. The miniaturized method was compared with the macro volume determination. The presence of oxygen in micro volumes presents the biggest obstacle for the miniaturized method.