Salvianolic Acid A Inhibited Angiotensin II-Induced Proliferation of Vascular Smooth Muscle Cells by Restraining Src and Akt Phosphorylation

The purpose of this study is to investigate the inhibitive effect of salvianolic acid A (SAA) on angiotensin II-induced proliferation in rat aortic vascular smooth muscle cells (VSMCs) and the possible mechanisms. The objects of study are divided into control group, angiotensin II group, SAA group and angiotensin II + SAA group (12.5, 25, 50, 100, and 200 mu M). Compared with control group, angiotensin II could significantly increase the proliferation of VSMCs and SAA markedly decreased the quantity of VSMCs. The levels of p-Src, p-Akt308 and p-Akt473 expression in angiotensin II + SAA group were significantly lower than those of angiotensin II group in a concentration-dependent fashion. In addition, with the extension of time, the expressions of p-Src, p-Akt 308 and p-Akt 473 were significantly lower than those of angiotensin II group in a time-dependent fashion. SAA could inhibit angiotensin II-induced proliferation of VSMCs in concentration and time-dependent fashions, the effects were carried out through decreasing the activation of p-Src and p-Akt.