Microscopy-Based Assay For Semi-Quantitative Detection Of Sars-Cov-2 Specific Antibodies In Human Sera A Semi-Quantitative, High Throughput, Microscopy-Based Assay Expands Existing Approaches To Measure Sars-Cov-2 Specific Antibody Levels In Human Sera
BIOESSAYS(2021)
摘要
Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-CoV-2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi-automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS-CoV-2 serological assays. Sensitive, specific and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high-throughput microscopy to rapidly develop serological assays for emerging virus infections.
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关键词
antibody, immunofluorescence, machine learning image analysis, quantitative microscopy, SARS‐, CoV‐, 2, serological test
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