Highly Convenient And Highly Specific-And-Sensitive Pcr Using Se-Atom Modified Dntps
CHEMICAL COMMUNICATIONS(2021)
摘要
Primer design and condition optimization for PCR are tedious and labour-intensive. To conveniently achieve high selectivity, sensitivity and robustness, herein, we first report a new strategy with Se-dNTPs to enhance PCR specificity (over 240-fold) and sensitivity (up to single-digit), effectively eliminating non-specific products and simplifing PCR design and optimization.
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