Chemical Composition of Fruits and Flowers from Clusia nemorosa G. Mey. (Clusiaceae)

The species Clusia nemorosa is popularly known as "camacari". The flowers are visited by bees, mainly native and the floral resin is used for the construction of nests, being the polyisoprenylated benzophenones the main components of this resin. The present work aimed to analyze the chemical constituents of the flowers and fruits of Clusia nemorosa. The flowers and fruits were extracted with ethanol. The ethanolic extract was subjected to extraction in solid phase with methanol. Analyzes of the methanolic fraction by ultra-performance liquid chromatography coupled with a diode array detector and quadrupole time-of-flight mass spectrometry (UPL das benzofenonas prenilados detectados C-DAD-qTOF-MS) and combined with the use of data-independent acquisition (MSE) for the profiling and structural characterisation of the compounds. The compounds were identified as condensed tannins, steroids and isomers of prenylated benzophenones. The chemical profile when compared of flowers and fruits showed little variation. The analysis of the high resolution mass spectra allowed to identify, classify and separate the two main types of nuclei from the prenylated benzophenones present in the flowers and fruits of Clusia nemorosa.