Extraction Of Antidandruff Compound From Lawsonia inermis.

The present research focused to, evaluate the utilization of henna leaves extract as sources of natural anti-dandruff compounds. Malassezia furfur (M. furfur) isolated from scalp of subjects and medium standardization was done. The growth curve of the fungus was observed. Henna extracts were prepared using different solvents. Antifungal activity of various crude extracts were detected by well and disc diffusion methods. Comparison on various concentration of henna extracts and known ketoconazole compound stabilized by BHT (an antioxidant) were studied. Partial fractionation of the crude extract was done by Thin layer chromatography (TLC) followed by characterizing the maximal antifungal activity compound by (HNMR)-H-1 and (CNMR)-C-13. Microscopic examination revealed dermatophytes and presence of small, smooth, pale white colonies on selective media. Growth curve were observed approximately 65 hrs of inoculation. The zone of inhibition was observed by 60% hexane extracts of Henna leaves by Disc diffusion method. The Ketoconazole was also stabilized by BHA and respective activity was found to beincreased. TLC analysis showed the presence of similar compound in NMR revealed that the imidazole was found to resemble Ketoconazole. The air-dried, powdered leaves of Lawsonia inermis (L.inermis) were used with the different solvents to observe the antifungal activity against the dandruff-causing dermatophyte, M. furfur. The results were then compared with the standard anti-dandruff compound ketoconazole with and without antioxidant stabilization. The extracted anti-dandruff compound is then characterized with the help of NMR Spectroscopy