Partial Purification of Phytase and Mannanase from Lactobacillus plantarum and Kinetic Determination of the Features of the L. plantarum immobilized onto the Magnetite Florisil Nanoparticle

In this study, production of the phytase and mannanase was carried out in a natural medium which includes corn (C) and wheat (W) using free and immobilized L. plantarum bacterial strains. Accordingly, test bacteria were inoculated into media containing C (10, 20, 30, 40 gr) and W (10, 15, 20 gr) at different ratios and left to incubate at the growth conditions pH 6.0-6.5 and 35 degrees C. First, -NH2 group was attached to the support material using APTES. Then, the sub-branch was formed using glutaraldehyde and the resulting Schiff was reduced with sodium borohydride to make the basic compound stable. Activated support material nano florisil was made magnetic with Fe3O4 compound and used for the immobilization of the bacteria. It was determined that approximately 50% of the prepared bacteria immobilized to the nanoparticles of magnetite florisil. The production of phytase and mannanase was performed with both free microorganisms and microorganisms attached to the matrix phytase and mannanase production by L. plantarum using corn and wheat natural media increased by 10-15% compared to those of the free isolates. In light of these findings, it was concluded that magnetite florisil nanoparticles can be used in many areas especially food and agriculture.