In vitro and computational studies of the antioxidant and anti-diabetic properties of Bridelia ferruginea

The leaves, stem and root bark of Bridelia ferruginea were sequentially extracted with solvents of increasing polarity to yield the hexane, ethyl acetate, ethanol and aqueous extracts. In vitro analysis revealed the ability of the extracts to scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide (NO) and hydroxyl radical. They also inhibited the activities of alpha-glucosidase, alpha-amylase and lipase enzymes. Gas chromatography-mass spectroscopic (GC-MS) analysis of the extracts revealed the presence of sterols, aromatics, aliphatic acids and esters. The identified compounds were molecularly docked with alpha-glucosidase, alpha-amylase and lipase enzymes. All compounds showed good binding affinities with the enzymes studied. The strongest binding affinities were observed for beta-amyrin, 4-phenylbenzophenone and lupenone for alpha-glucosidase, alpha-amylase and lipase enzymes, respectively. The data suggest antioxidant and antidiabetic potential of the different parts of B. ferruginea, with the leaves having the highest potential. These properties can be explored for development of novel anti-diabetic drugs. Communicated by Ramaswamy H. Sarma