Improvement of the 25-hydroxyvitamin D3 production in a CYP109A2-expressing Bacillus megaterium system

Ammar Abdulmughni, Björn Erichsen, Jürgen Hensel,Frank Hannemann,Rita Bernhardt

Journal of Biotechnology（2021）

引用 6|浏览6

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摘要

•Demonstrating the importance of the region between amino acids T103-A106 of CYP109A2 for the hydroxylation of vitamin D3.•Increasing the productivity of CYP109A2-based whole-cell system by optimizing the reaction conditions.•Using the mutant T103A under optimized conditions resulted in a 5-fold higher amount of 25(OH)VD3 compared to the wild type.

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关键词

Calcifediol,Bacillus megaterium,Cytochrome P450,Putative redox-partner binding site,Site-directed mutagenesis

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