Exploring the biochemical properties of three polyphenol oxidases from blueberry (Vaccinium corymbosum L.)

Yulong Wei,Ning Yu,Yue Zhu,Jingyi Hao,Junyan Shi,Yuqing Lei,Zhilin Gan,Guoliang Jia,Chao Ma,Aidong Sun

Food Chemistry（2021）

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摘要

•Three blueberry PPOs were successfully purified for the first time.•Three purified blueberry PPOs were identified by LC-MS/MS analysis.•The protein sequence of blueberry PPO (CUFF.45663.1) core domain was deduced.•Partial enzymatic properties of three purified blueberry PPOs were determined.•The binding of substrates for blueberry PPOs was related to the hydrogen bond.

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关键词

Blueberry,PPOs,Purification,Biochemical properties,Binding mechanism

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