Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15 degrees C

A total of 30 ejaculates comprising five from each of six boars (three Hampshire and three Rani breeds) were collected to study the effect of DMSO (Dimethyl sulfoxide) on preservability and fertility of boar semen at liquid state at 15 degrees C in ten extenders viz. Androheps+Strepto-penicillin+Gentamycin, GEPS+Strepto-penicillin+Gentamycin, Androheps + 1% DMSO, Androheps + 0.7% DMSO, Androheps + Gentamicin sulfate + 0.5% DMSO, Androheps +Strepto-penicillin + 0.5% DMSO, GEPS (Glucose-sodium salt of EDTA-potassium sodium tartrate-sodium citrate dihydrate) + 1% DMSO, GEPS+0.7% DMSO, GEPS+Gentamicin sulfate+0.5%DMSO and GEPS+ Strepto-penicillin + 0.5% DMSO extenders. After holding semen each ejaculate was split into 10 parts and extended (1:3) with the said experimental dilutors and preserved at 15 degrees C in refrigerator up to 168 hr. The evaluation of sperm motility and Hypo-osmotic swelling test (HOST) of preserved semen samples were recorded at 0 (just after extension), 24, 48, 72, 96, 120, 144 and 168 hr as per the standard method. The results indicated that the addition of DMSO in extenders had no significant effect on preservability of boar semen at 15 degrees C.