Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization.
European journal of dentistry(2020)
摘要
OBJECTIVE: This study aims to confirm whether the GDMSCs isolated from rabbit's (
Oryctolagus cuniculusMATERIALS AND METHODS: This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (
O. cuniculusnRESULTS: GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits
in vitroCONCLUSION: GDMSCs isolated from the rabbits (
O. cuniculusin vitroin vitro
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