Establishment and evaluation of a murine alpha v beta 3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus

Integrin alpha v beta 3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of alpha v beta 3 integrin, a stable CHO-677 cell line expressing the murine alpha v beta 3 heterodimer (designated as "CHO-677-m alpha v beta 3" cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits alpha v and beta 3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-m alpha v beta 3 cell line at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed stably. Aplaque-forming assay, 50% tissue culture infective dose (TCID50), real-time quantitative reverse transcription-PCR, and IFA were used to detect the replication levels of Foot-and-mouth disease virus (FMDV) in the CHO-677-m alpha v beta 3 cell line. After infection with FMDV/O/ZK/93, the cell line showed a significant increase in viral RNA and protein compared with CHO-677 cells. These findings suggest that we successfully established a stable alpha v beta 3-receptor-expressing cell line with increased susceptibility to FMDV. This cell line will be very useful for further investigation of alpha v beta 3 integrin, and as a cell model for FMDV research.