Continuous-Flow Synthesis of beta-Amino Acid Esters by Lipase-Catalyzed Michael Addition of Aromatic Amines

A continuous-flow procedure for the synthesis of beta-amino acid esters has been developed via lipase-catalyzed Michael reaction of various aromatic amines with acrylates. Lipase TL IM from Thermomyces lanuginosus was first used to catalyze Michael addition reaction of aromatic amines. Compared with other methods, the salient features of this work include green reaction conditions (methanol as reaction medium), short residence time (30 min), readily available catalyst and a reaction process that is easy to control. This enzymatic synthesis of beta-amino acid esters performed in continuous-flow microreactors is an innovation that provides a new strategy for the fast biotransformation of beta-amino acid esters.