Spectrophotometric Detenmination of Spermine with Vanillylfluorone and Cobalt(II)

A novel spectrophotometric method was established for the determination of speimine based on color complex formation among vanillylfluorone, cobalt(II) and spermine in the presence of stearyltrimethylammonium chloride. In the determination of spermine, Beer's low was obeyed in the range of 0.04 - 1.0 mu g mL(-1.) The effective molar absorptivity at 615 nm and the relative standard deviation were 2.2 X 10(5) L mol(-1) cm(-1) and 2.5 % (n = 5), respectively.