Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS
BIO-PROTOCOL(2017)
摘要
Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen (Keren et al., 2002). In this protocol, we describe a method to quantify the periplasmic and intracellular pools of Mn in Synechocystis accurately, using inductively coupled plasma mass spectrometry (ICP-MS).
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关键词
Cyanobacteria,Synechocystis,Manganese,Periplasm,ICP-MS
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