MICROFLUIDIZATION-ASSISTED EXTRACTION OF POLYSACCHARIDE FROM EUCOMMIA ULMOIDES OLIV. LEAVES AND EVALUATION OF ITS ANTIOXIDANT ACTIVITY

Orthogonal test was used to optimize the process conditions of microfluidization-assisted extraction (MAE) of polysaccharide from Eucommia ulmoides Oliv. leaves. The effects of pressure, temperature and the time of extraction on the yields of polysaccharide and their antioxidative effect were investigated. Based on the single factor experiments, the optimal extraction conditions were as follows : pressure 200 MPa, extraction temperature of 60 degrees C and time extraction 60 min. Under these conditions, the polysaccharide yield was 1.86%. E. ulmoides leaves polysaccharide (EP) has a better scavenging effect on DPPH radicals and hydroxyl free radicals than ascorbic acid (V-C). MAE is a suitable method for extraction of polysaccharide from E. ulmoides leaves.