A Chimeric Gene Paternally Instructs Female Sex Determination In The Haplodiploid Wasp Nasonia

Various primary signals direct insect sex determination. In hymenopteran insects, the presence of a paternal genome is needed to initiate female development. When absent, uniparental haploid males develop. We molecularly and functionally identified the instructor sex-determination gene, wasp overruler of masculinization (worn), of the haplodiploid wasp Nasonia vitripennis. This gene contains a P53-like domain coding region and arose by gene duplication and genomic rearrangements. Maternal silencing of worn results in male development of haploid embryos. Upon fertilization, early zygotic transcription from the paternal worn allele is initiated, followed by a timely zygotic expression of transformer (tra), leading to female development. Worn is an instructor gene with a parent-of-origin effect in sex determination.