miR‑576‑3p overexpression enhances cisplatin sensitivity of ovarian cancer cells by dysregulating PD‑L1 and cyclin D1.

Cisplatin (DDP) resistance is a major obstacle in the chemotherapeutic efficacy of ovarian cancer. The present study aimed to explore the role of miR-576-3p in DDP sensitivity of ovarian cancer cells. Ovarian cancer cell lines SKOV3 and A2780 and DDP-resistant ovarian cancer cell lines SKOV3/DDP and A2780/DDP were used in the present study. In vitro studies demonstrated that microRNA (miR)-576-3p overexpression increased the DDP sensitivity of DDP-resistant ovarian cancer cells. A dual-luciferase assay verified that both programmed death-ligand 1 (PD-L1) and cyclin D1 were targets of miR-276-3p and were reversely associated with the expression of miR-576-3p. Moreover, in vivo studies indicated that tumorigenesis was inhibited by DDP, which was enhanced by further miR-576-3p overexpression in tumor tissues. Taken together, the results suggested that miR-576-3p overexpression increased DDP chemosensitivity of ovarian cancer cells via decreasing PD-L1 and cyclin D1, indicating that miR-576-3p may serve as a promising therapeutic target for ovarian cancer.