Biomarkers, matrices and analytical methods targeting human exposure to chemicals selected for a European human biomonitoring initiative

•The EU Horizon 2020 project HBM4EU targets seven substance groups and two metals.•Best suitable exposure biomarkers, matrices and analytical methods were suggested.•The selection was based on generally applicable criteria, e.g. method sensitivity.•Some conflicts were identified between the criteria and common exposure biomarkers.•Stringent quality assurance and control measures should be included and reported.