Chrysalises as natural production units for recombinant subunit vaccines

•Trichoplusia ni chrysalises contain million of insect cells in perfect physiological conditions that can be programmed by a baculovirus vector.•CrisBio technology uses chrysalises as natural bioreactors in combination with baculovirus vectors to produce recombinant proteins.•CrisBio is an advanced semi-automated non-fermentative insect-based technology able to produce dozens of vaccine doses per infected chrysalis.