In vivo hypoxia characterization using blood oxygen level dependent magnetic resonance imaging in a preclinical glioblastoma mouse model

•BOLD MRI can be optimized to identify hypoxic murine glioblastoma tumors.•BOLD MR images can identify sub-regions of hypoxia within the tumor.•Hypoxia co-localization between pimonidazole IHC and BOLD identified.•In-house MATLAB code was developed to train and validate BOLD MRI methodology.