Zelkovamycins B-E, Cyclic Octapeptides Containing Rare Amino Acid Residues From An Endophytic Kitasatospora Sp

Four unusual cyclopeptides, zelkovamycins B-E (1-4), were isolated from an endophytic Kitasatospora sp. Zelkovamycin B was featured by an unprecedented 3-methyl-5-hydroxypyrrolidine-2,4-dione ring system linked to the cyclopeptide skeleton. Their structures and full configurations were established by spectroscopic analysis, Marfey's method, and NMR calculations. A plausible biosynthetic pathway for zelkovamycins was proposed based on gene duster analysis. Zelkovamycin E displayed potent inhibitory activity against H1N1 influenza A virus.