USE OF Mangifera indica L. LEAVES EXTRACT AND MANGIFERIN ON THE ATHEROSCLEROTIC LESION IN APOE-/- MICE.

Mangifera indica L leaf are an important source of phenolic compounds, especially mangiferin, that exhibits antidiabetic, hypolipidemic, anti-oxidant and anti-inflammatory activities. This study aimed to evaluate the effects of mangiferin and ethanolic extract of M. indica leaf on atherosclerotic lesions in mice ApoE(-/-). Methods: Fifteen-week-old ApoE(-/-) mice were randomly divided into 4 groups according to the treatment giving by gavage during 56 days: control - vehicle (dimethyl sulfoxide); E200 - 200 mg/kg/day M. indica leaf extract; E400 - 400 mg/kg/day M. indica leaf extract, M40 - 40 mg/kg/day mangiferin. Administrations of vehicle, extracts and mangiferin were performed every day by gavage during 8 weeks. Blood parameters were measured using enzymatic kits and atherosclerotic lesions were evaluated by en face method. Results: The dired extract showed 17% of mangiferin. Total cholesterol, HDLc, LDLc and triglycerides blood levels, as well as the percentage of lipid deposition in the aortic arch and thoracic aorta were not significantly different between the groups (p> 0.05). Conclusion: The administration of M. indica leaf extract and mangiferin in ApoE(-/-) mice did not affect serum lipids and did not decreased pre-existing atherosclerotic lesions