Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 mu g/L (835 mu g/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25 degrees C, pH(initial) 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h(-1) of maximum specific growth speeds (mu(max)) was obtained for the major carotenoid - (all-E)-beta-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the beta-carotene.