The whole mitochondrial genome of Sarcophaga kanoi (Diptera: Sarcophagidae)

MITOCHONDRIAL DNA PART B-RESOURCES(2020)

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摘要
Sarcophaga kanoiPark, 1962, a widely distributed flesh fly in Southeast Asia, is important in forensic entomology. Notably, its mitochondrial genome could provide the unique and accurate molecular information in species identification which facilitate forensic practices in estimation of postmortem interval especially in putrefied cadaver cases. Thus, we sequenced and characterized the whole mitochondrial genome (mitogenome) ofS. kanoifor the first time, which was collected from Southern China in this study. The 15,319 bp mitogenome contained 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and a putative control region. The total nucleotide composition of this circular genome was 39.7% for A, 9.3% for G, 14.2% for C, and 36.8% for T. To better understand the genetic relationship, the phylogenetic analysis was constructed according to the 13 PCGs sequence ofS. kanoiand other 11 species. The phylogenetic tree showed that theS. kanoiwas placed in a sub-clade withS. similis. Our study updated the new genetic information for dipteran mitogenomes, which could broaden the background knowledge for forensic entomology, molecular genetics and developmental biology. It has the potential application on the species identification using genetic markers.
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关键词
Sarcophagidae,Sarcophaga kanoi,mitochondrial genome
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