Strain improvement of long-chain fatty acids producing Micractinium sp. by flow cytometry

Deepi Deka,Riwandahun Marwein,Channakeshavaiah Chikkaputtaiah,Shiva Shanker Kaki,Thirupathi Azmeera,Hari Prasanna Deka Boruah,Natarajan Velmurugan

Process Biochemistry（2020）

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摘要

•Bioprocess of long-chain fatty acids accumulating new isolate Micractinium sp.•Optimized FACS conditions in combination with fluorescent dyes for cell sorting.•Established of FACS-based adaptive evolution strategy for strain improvement.•Achieved improved phenotypes with high biomass and desirable fatty acids. composition

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关键词

Long-chain fatty acids,Micractinium sp.,Strain improvement,Fluorescent activated cell sorter,Adaptive evolution

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