Antigen-specific human CD4+T cell clones as tools to characterize immunomodulatory receptor antibodies for immunotherapy.

T-cell activation requires co-stimulatory signals that are provided by the interaction between Immuno-Modulatory Receptor (IMR) receptors and ligands expressed on the T cells and APC or tumor cells. Hence, an antigen (Ag)-specific human T-cell clone system was developed. It has been applied not only to study the biology of IMRs, but also to characterize the different biologics targeting those molecules as single agents or in combination to test improved efficacy. Data shown will include the establishment of alloreactive human CD4+ T-cell clones, FACS profiling IMRs of T-cell clones, engineering of different antigen presenting cells transfected with IMR ligands, and application of T-cell clones in characterization of anti-PD1 and anti-Lag3 antibodies Citation Format: Sophie (Ying) Li, Sabine Le Saux, Robert Shields, Rene de Waal Malefyt. Antigen-specific human CD4+ T cell clones as tools to characterize immunomodulatory receptor antibodies for immunotherapy [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2018 Nov 27-30; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(4 Suppl):Abstract nr A96.