Green Synthesis of Reduced Graphene oxide Nanosheets Using Leaf Extract of Lantana camara and Its In-Vitro Biological Activities

In recent years, sustainable green chemistry sets eco-efficiency approaches to synthesis next generation nanomaterials using plant sources as reducing agents. Among them, reduced Graphene oxide (rGO) has been developing as biocompatible nanomaterials in biomedicine. The present work deals with facile synthesis of rGO using leaf extract of Lantana camara (LC). The bioreduction of graphene oxide (GO) to reduced Graphene oxide by L. camara (LCrGO) was confirmed by spectroscopic techniques such as UV–visible, XRD, FTIR, Raman spectrometer, Zeta potential and DLS. SEM and TEM microscopic revealed the presence of thin nano sheets of LCrGO. EDX detected intense signals of Carbon (86%) and Oxygen (13%) present in LCrGO. The bioreduced LCrGO showed potential in vitro biological effects. DPPH assay showed 79% radical inhibition at 500 µg/ml. The LCrGO exhibited enhanced bactericidal effects against B. subtilis (16 mm) and E. coli (12 mm) at 500 µg/ml. MTT assay showed concentration dependent cytotoxic effects against lung cancer cell lines (A549) with decrease in cell viability (44%) at maximum concentration of LCrGO (500 µg/ml). From the observations, it is concluded that rGO nanosheets synthesized using L. camara could be used for formulation of nanomedicine.