Detection of Genetic Variations in Pattern Recognition Receptors (PRRs) Gene of Tharparkar Cattle Breed

The present study was carried out to explore polymorphic patterns in the complete coding sequences of Toll-like receptor2 (TLR2) gene in Tharparkar cattle breed originating in Tharparkar district in Sindh province. Complete TLR2 gene [5'UTR 136bp, CDS 2355bp and 3'UTR 1316bp] was sequenced encoding 784 amino acids. Out of 14 variants observed in CDS, 43% were non-synonymous (NS) and 57% were synonymous (Syn). Phylogenetic analysis revealed clustering of Tharparkar breed with Bos indices as the nearest neighbor. The substitution at amino acid position R to H was assumed to have possible damaging functional effect. The predicted 3D structure of bovine TLR2 is a solenoid-like structure containing the active sites that form binding pocket for ligand. Thus, our findings suggest that the genetic variations of TLR2 gene providing important genetic insight into disease resistance in Tharparkar cattle. (C) 2020 PVJ. All rights reserved