BtpA and BtpB TIR filaments are not coincident with yeast tubulin.

Julia María Coronas-Serna, Arthur Louche, María Rodríguez-Escudero, Morgane Roussin, Paul R. C. Imbert,Isabel Rodríguez-Escudero,Laurent Terradot, María Molina,Jean-Pierre Gorvel,Víctor J. Cid,Suzana P. Salcedo

PLOS Pathogens（2020）

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摘要

Indirect immunofluorescence of YPH499 yeast cells expressing GFP-BtpA-TIR, GFP-BtpB-TIR, and their corresponding E234A mutant versions from pYES2 plasmid derivatives (green). Microtubules are stained using anti-tubulin antibody (red). Nuclei are labelled with DAPI (blue). Scale bars correspond to 5 μm. (PDF)

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