Modeling host-feeding preference and molecular systematics of mosquitoes in different ecological niches in Canada

•This is the first work to analyze the host-feeding pattern of different mosquito species in Canada via molecular techniques.•We found 34 different mosquito taxa in Canada by combining morphological and molecular identification approaches.•Our data showed that the 201 blood-fed mosquitos were spread over 20 taxa who fed on 10 mammalian species, and three bird species.•The majority of all analysed mosquito taxa fed on humans (24.3%) followed by pigs (21.8%).•In our study, seven mosquito species with both ornithophilic and anthropophilic biting habit could function as potential bridge vectors.