In vivo modification of the goat mammary gland glycosylation pathway

María J. Leiva-Carrasco,Silvana Jiménez-Chávez,David J. Harvey,Natalie C. Parra,Kaio C. Tavares,Frank Camacho,Alain González,Oliberto Sánchez,Raquel Montesino,Jorge R. Toledo

New Biotechnology（2021）

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摘要

•In vivo genetic modification of glycosylation pathway of goat mammary gland.•Mammary gland as bioreactor for the production of complex recombinant glycoproteins.•In vivo adenoviral transduction enhances the co-expression of recombinant proteins.

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EPO,rhEPO,EPO-Fc,GnT-IVa,EPO-Fc/GnT-IVa,FCS,DMEM,MGAT4A,Ad,MOI,GFP,SDS-PAGE,PNGase F,2-AB,NP-HPLC,MS,ESI,CID

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