Lc-Ms/Ms Quantification Of Asymmetric Dimethyl Arginine And Symmetric Dimethyl Arginine In Plasma Using Surrogate Matrix And Derivatization With Fluorescamine
BIOANALYSIS(2020)
摘要
Aim: A novel LC-MS/MS method using a surrogate matrix and derivatization with fluorescamine was developed and validated for simultaneous quantification of asymmetric dimethyl arginine and symmetric dimethyl arginine. Methods & results: Asymmetric dimethyl arginine, symmetric dimethyl arginine and corresponding internal standards were extracted using protein precipitation and derivatization with fluorescamine followed by SPE. Derivatives were analyzed by turbo ion spray LC-MS/MS in the positive ion mode. Methodology was successfully transferred across multiple preclinical species and utilized in the support of several investigative studies. Conclusion: A new LC-MS/MS analytical methodology that utilizes a surrogate matrix and derivatization with fluorescamine was successfully developed and validated.
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关键词
ADMA, derivatization, fluorescamine, parallelism, SDMA
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