Human Lymphoid Stromal Cells Contribute To Polarization Of Follicular T Cells Into Il-4 Secreting Cells

Fibroblastic reticular cells (FRCs) are the specialized lymphoid stromal cells initially identified as triggering T-cell recruitment and dynamic motion in secondary lymphoid organs. Interestingly, FRCs also display antigen presentation capacities and support lymphocyte survival. CXCR5(+)CD4(+)follicular T cells are important players of B-cell maturation and antibody response. Our study reported thatin vitro-differentiated FRC-like cells enhanced the growth of the whole CXCR5(+)CD4(+)T-cell compartment, while enhancing IL-4 secretion specifically by the PD1(dim)CXCR5(+)CD4(+)cell subset, in a Notch- and ICAM1/LFA1-dependent manner. In addition, we revealed that in follicular lymphoma (FL) tissues, previously identified as enriched for PD1(hi)CXCR5(hi)CD4(+)mature follicular helper T cells, PD1(dim)CXCR5(+)CD4(+)T cells displayed an enrichment for Notch and integrin gene signatures, and a Notch and ICAM-1-dependent overexpression of IL-4 compared to their non-malignant counterparts. These findings suggest that the crosstalk between FRCs and CXCR5(+)PD1(dim)CD4(+)T cells may contribute to the FL IL-4 rich environment, thus providing new insights in FL lymphomagenesis.