Complementary experimental and computational analysis of the effects of non-ionic detergents and phospholipids on insulin amyloid aggregation

•Amyloidogenesis of insulin is significantly affected by DDM in a time-and dose-dependent manner.•Amyloidogenesis of insulin only slightly affected by studied phospholipids (PLs).•DDM and the PLs occupy equivalent binding sites; however, the resulting effect on insulin aggregation is noticeably different.•Detergents and phospholipids are not always interchangeable in their use to investigate protein amyloid aggregation.•Hydrogen bonds indicated from MD simulations is the decisive factor for larger anti-aggregation effect of DDM over the PLs.