Shanghaineisseria Gonorrhoeaeisolates Exhibit Resistance To Extended-Spectrum Cephalosporins And Clonal Distribution

The emergence ofNeisseria gonorrhoeaestrains with resistance (R) to extended-spectrum cephalosporins (ESCs(R)) represents a public health threat of untreatable gonococcal infections. This study was designed to determine the prevalence and molecular mechanisms of ESC(R)of ShanghaiN. gonorrhoeaeisolates. A total of 366N. gonorrhoeaeisolates were collected in 2017 in Shanghai. Susceptibility to ceftriaxone (CRO), cefixime (CFM), azithromycin (AZM), ciprofloxacin (CIP), spectinomycin, penicillin, and tetracycline was determined using the agar dilution method. A subset of 124 isolates was subjected to phylogenetic analysis for nine antimicrobial resistance-associated genes, i.e.,penA,porB,ponA,mtrR, 23S rRNA,gyrA,parC, 16S rRNA, andrpsE. Approximately 20.0% of the isolates exhibited CFM(R)[minimum inhibitory concentration (MIC) >0.125 mg/L], and 5.5% were CRO(R)(MIC > 0.125 mg/L). In total, 72.7% of ESC(R)isolates were clonal and associated with mosaicpenA10 and 60 alleles. Non-mosaicpenA18 allele and substitutions of PenA A501T, G542S, and PorB1b G213S/Y were observed in non-clonal ESC(R). Approximately 6.8% of the isolates showed AZM MIC above the epidemiological cutoff (ECOFF, 1 mg/L), were associated with 23S rRNA A2059G mutation, and did not exhibit clonal distribution. Almost all isolates were CIP(R)(resistance to ciprofloxacin) and associated with GyrA-91/92 and ParC-85/86/87/88/89/91 alterations. Isolates with ParC S88P substitution were clustered into the ESC(R)clade. The Shanghai isolates exhibited a high level of ESC(R)and distinct resistant patterns.